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Gomez, JAuthor

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September 27, 2022
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Article

Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity

Publicated to: Redox Biology. 34 (101539): 101539- - 2020-07-01 34(101539), DOI: 10.1016/j.redox.2020.101539

Authors:

Mota-Martorell, N; Jove, M; Pradas, I; Sanchez, I; Gómez, J; Naudi, A; Barja, G; Pamplona, R
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Affiliations

Univ Complutense Madrid, Dept Genet Physiol & Microbiol, Madrid, Spain - Author
Univ Lleida, Dept Expt Med, Lleida Biomed Res Inst UdL IRBLleida, Biomed 1 Bldg,Av Rovira Roure 80, Lleida 25198, Catalonia, Spain - Author
Univ Lleida, Prote & Genom Unit, Lleida, Catalonia, Spain - Author
Univ Rey Juan Carlos I, Dept Biol & GeolPhys & Inorgan Chem, ESCET Campus Mostoles, Madrid, Spain - Author
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Abstract

Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species -specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage -dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.
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Keywords

AdultAgingAnimalAnimal tissueAnimalsArticleCaloric restrictionComplex iControlled studyCowCytochrome-cDroplet digital pcrDroplet digital polymerase chain reactionElectron transportElectron transport complex iFree-radical productionGene expressionGenerationGeneticsGuinea pigHeart tissueHeart-mitochondriaHorseHydrogen-peroxide productionHydrophilicityLeporidaeLongevityMaleMammalMammalsMetabolismMitochondriaMitochondrial permeabilityMitochondrial proteinMitochondrial ros productionMitochondrionMouseNdufs4 subunitNdufv2 subunitNonhumanOxidative stressPermeability transitionPhenotypePredictionPriority journalProteinProtein contentProtein domainProtein ndufa9Protein ndufs3Protein ndufs4Protein ndufs5Protein ndufv2Protein structureProteomicsRatReactive oxygen metaboliteReactive oxygen speciesReduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone)Species differenceUnclassified drugVdacVoltage dependent anion channel 1Western blotWestern blotting

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Redox Biology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2020, it was in position 21/295, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.07. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.2 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-05, the following number of citations:

  • WoS: 29
  • Scopus: 29
  • Europe PMC: 17
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 79.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 79 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 17.
  • The number of mentions on the social network Facebook: 1 (Altmetric).
  • The number of mentions on the social network X (formerly Twitter): 10 (Altmetric).
  • The number of mentions in news outlets: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Awards linked to the item

This work was supported by the Spanish Ministry of Economy and Competitiveness, Institute of Health Carlos III (grant number PI14/00328), the Spanish Ministry of Science, Innovation and Universities (RTI2018-099200-B-I00), and the Generalitat of Catalonia, Agency for Management of University and Research Grants (2017SGR696) and Department of Health (SLT002/16/00250) to R.P, and PR [19] BIO MET 0155 to GB.This study has been co-financed by FEDER funds from the European Union (A way to build Europe). IRBLleida is a CERCA Programme/Generalitat of Catalonia.
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