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Ros, MAuthorMartinez, CAuthor
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ObRa and ObRe are differentially expressed in adipose tissue in aged food-restricted rats: Effects on circulating soluble leptin receptor levels

Publicated to:Endocrinology. 146 (11): 4934-4942 - 2005-11-01 146(11), DOI: 10.1210/en.2005-0220

Authors: Gallardo, N; Arribas, C; Villar, M; Ros, M; Carrascosa, JM; Martínez, C; Andrés, A

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Abstract

In rodents, soluble leptin receptor (SLR) may be generated by alternative splicing of ObR mRNA and/or as a cleavage product of ObR membrane-anchored receptors. In this study, we investigated the contribution of both processes on the generation of SLR in 3-, 8-, and 24-month-old Wistar rats fed ad libitum (AL) or under food restriction (FR). To this end, we determined serum SLR levels and analyzed ObRa and ObRe mRNA expression under these physiological conditions. Additionally, we studied the cellular distribution of ObRa and the generation of SLR by N-ethyl-maleimide-induced shedding from ObRa membrane receptors in isolated adipocytes. Serum SLR levels were significantly increased in 8- and 24-month-old rats under FR, whereas similar amounts were found in rats of different ages fed AL. ObRa and ObRe mRNA expression in epididymal adipose tissue increased with aging. In contrast, after FR, ObRe mRNA expression decreased, whereas ObRa mRNA expression further increased compared with 8- and 24-month-old rats fed AL. Additionally, FR promoted a change in the distribution of ObRa between internal and plasma membranes in isolated adipocytes, increasing its presence at the cell surface. Finally, the generation of SLR by N-ethyl-maleimide-induced shedding from ObRa was also increased under FR. These data suggest that shedding of ObRa membrane-anchored receptors, rather than ObRe expression, might preferentially contribute to the generation of the increased levels of SLR in serum under conditions of FR.

Keywords
AdipocyteAdipocytesAdipose tissueAgingAnimal cellAnimal experimentAnimal nutrition physiologyAnimal nutritional physiological phenomenaAnimal tissueAnimalsArticleBound leptinCaloric restrictionCell isolationCell membraneCell surfaceCellular distributionCloningControlled studyDiet restrictionEpididymisEpididymis fatEthylmaleimideGenerationHormonesIdentificationIncreasesLeptin receptorMaleMembrane receptorMessenger rnaMetabolismModulationMutationN ethylmaleimideNonhumanNucleotide sequenceObese geneObra proteinObre proteinPriority journalProtein expressionProtein isoformsRatRat strainRatsRats, wistarReceptors, cell surfaceReceptors, leptinRna, messengerSolubilitySubcellular fractionsSulfhydryl reagentsTissue distributionUnclassified drug

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Endocrinology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2005, it was in position 12/89, thus managing to position itself as a Q1 (Primer Cuartil), in the category Endocrinology & Metabolism.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.09, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-22, the following number of citations:

  • WoS: 17
  • Scopus: 24
  • Europe PMC: 12
  • OpenCitations: 22
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-22:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 25 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.