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The financial support of CTQ2015-64402-C2-1-R (Spanish Ministerio de Economia y Competitividad), RTI2018-096135-B-I00 (Spanish Ministerio de Ciencia, Innovacion y Universidades) and the TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid (Grant S2018/NMT-4349) are gratefully acknowledged.

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Martínez-García, GonzaloAuthor

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October 18, 2022
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Electrochemical biosensor for the simultaneous determination of rheumatoid factor and anti-cyclic citrullinated peptide antibodies in human serum

Publicated to:Analyst. 145 (13): 4680-4687 - 2020-07-07 145(13), DOI: 10.1039/d0an00481b

Authors: Guerrero, Sara; Sanchez-Tirado, Esther; Martinez-Garcia, Gonzalo; Gonzalez-Cortes, Araceli; Yanez-Sedeno, Paloma; Pingarron, Jose M

Affiliations

Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, Madrid 28040, Spain - Author

Abstract

This paper reports a dual electrochemical biosensor involving carboxylated- or neutravidin-functionalized magnetic microbeads and dual screen-printed carbon electrodes for the simultaneous determination of rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCPA) autoantibodies used as biomarkers for the detection of rheumatoid arthritis autoimmune disease. Sandwich-type biosensors involving Fc fragments of IgG Fc(IgG) and biotinylated cyclic cytrullinated peptide (CCP-biotin) to form CCP-biotin-Neutr-MBs for the specific immobilization of RF and CCPA, respectively, as well as conjugation with HRP-IgM and HRP-IgG for RF and CCPA, respectively, were prepared. Amperometric detection was performed at -0.20 Vvs.Ag pseudo-reference electrode using the H2O2/hydroquinone (HQ) system upon capturing the bioconjugates onto the corresponding working electrode (WE1 or WE2) of SPCdEs. The dual biosensor exhibits high sensitivity for RF and CCPA with LOD values of 0.8 and 2.5 IU mL(-1), respectively. The simultaneous determination can be completed in about two hours using a simple protocol and a sample volume (25 mu L) four times smaller than that required by the ELISA method. The dual electrochemical biosensor was used for the determination of both target biomarkers in human serum.

Keywords

Anti-citrullinated protein antibodiesAntibodies, immobilizedArthritisArthritis, rheumatoidAutoantibodiesBiomarkersBiosensing techniquesCarbonElectrochemical techniquesElectrodesHumansImmunoassayImmunoglobulin fc fragmentsLimit of detectionRheumatoid factor

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Analyst due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2020, it was in position 19/87, thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemistry, Analytical.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.35. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.16 (source consulted: FECYT Feb 2024)
  • Field Citation Ratio (FCR) from Dimensions: 3.85 (source consulted: Dimensions Aug 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-08-21, the following number of citations:

  • WoS: 26
  • Scopus: 28
  • Europe PMC: 8

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-21:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 39 (PlumX).