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This study was funded by grants from the following: the Comunidad de Madrid (project S2009/MAT-1472); the Ministerio de Economia y Competitividad, MINECO and FEDER: MAT201343299-R and CGL2016-79158-P); and PROMETEO/2017/039 Excellence in Research (Generalitat Valenciana, Spain). M.C. Matesanz is greatly indebted to Spanish Ministry of Education (MEC) for predoctoral fellowship.

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Chemical Characterization of the Lichen-Symbiont Microalga Asterochloris erici and Study of Its Cytostatic Effect on the L929 Murine Fibrosarcoma Cell Line

Publicado en:Processes. 9 (9): 1509- - 2021-09-01 9(9), DOI: 10.3390/pr9091509

Autores: Concepcion Matesanz, M; Villa-Carvajal, Mercedes; Linares, Javier; Morante-Zarcero, Sonia; Sierra, Isabel; Barreno, Eva; Catala, Myriam; Teresa Portoles, M

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New resources of food, pharmaceuticals or biotechnological products are needed. The huge biodiversity of aero-terrestrial lichen-symbiont microalgae belonging to the Chlorophyta group remains unexplored despite they present interesting features such as extreme stress tolerance and growth in water shortage. Appropriateness for human consumption demands the demonstration of the absence of toxic effects. In vitro biocompatibility of crude homogenates of axenic microalga Asterochloris erici, isolated from the lichen Cladonia cristatella, was analyzed after treatment of cultured L929 fibroblasts with different concentrations of microalgal homogenates. The microalgal protein content (37%) was similar to spirulina or soybean. Antioxidant capacity (10.6 +/- 0.6 mu mol TE/g WW) or phenolic content (7.5 +/- 0.5 mg GAE/g DW) were high compared to Chlorella. The results show that crude homogenates of A. erici do not induce cytotoxicity but seem to have some cytostatic effect inducing slight cell cycle alterations and intracellular reactive oxygen species (ROS) increase at the highest concentration. Carotenoid analysis demonstrates high contents of lutein (1211 mu g/g microalga DW), a xanthophyll with antioxidant and cytostatic properties in vivo and high commercial added value. These findings confirm that Asterochloris erici can be suitable for the development of alimentary or pharmaceutical applications and further in vivo animal testing. The cytostatic effects should be further investigated for antitumor agents.

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