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Open Access funding enabled and organized by Projekt DEAL. The project leading to this application has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 896938 to ET. The project was further supported by research, network, and infrastructure Grants to KW (Carlsberg Foundation Grant Nos. CF15-0946 and CF20-0458, Independent Research Fund Denmark Grant No. 1026-00285B, Ministry of Science and Higher Education Denmark Grant No. 0192-00074B), and by research Grants to NM (JSPS KAKENHI Grant Nos. 18H04894 and 20 K06754).

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Hooked on zombie worms? Genetic blueprints of bristle formation in Osedax japonicus (Annelida)

Publicado en:Evodevo. 15 (1): 7- - 2024-06-04 15(1), DOI: 10.1186/s13227-024-00227-1

Autores: Tilic, Ekin; Miyamoto, Norio; Herranz, Maria; Worsaae, Katrine

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Background This study sheds light on the genetic blueprints of chaetogenesis (bristle formation), a complex biomineralization process essential not only for the diverse group of bristle worms (annelids) but also for other spiralians. We explore the complex genetic mechanisms behind chaetae formation in Osedax japonicus, the bone-devouring deepsea worm known for its unique ecological niche and morphological adaptations. Results We characterized the chaetal structure and musculature using electron microscopy and immunohistochemistry, and combined RNAseq of larval stages with in-situ hybridization chain reaction (HCR) to reveal gene expression patterns integral to chaetogenesis. Our findings pinpoint a distinct surge in gene expression during the larval stage of active chaetogenesis, identifying specific genes and cells involved. Conclusions Our research underscores the value of studying on non-model, "aberrant" organisms like Osedax, whose unique, temporally restricted chaetogenesis provided insights into elevated gene expression across specific larval stages and led to the identification of genes critical for chaetae formation. The genes identified as directly involved in chaetogenesis lay the groundwork for future comparative studies across Annelida and Spiralia, potentially elucidating the homology of chaetae-like chitinous structures and their evolution.

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